Abby Buchwalter, PhD

ASST PROF IN RES-HCOMP

M_Cardiovascular Research Inst

[email protected]

We explore how the cell nucleus is built, specialized across cell types, and maintained over time to influence cellular identity. We are uncovering principles of nuclear organization and defining how the nucleus is disrupted by aging and disease.

Publications

C-terminal tagging, transmembrane domain hydrophobicity, and an ER retention motif influence the secretory trafficking of the inner nuclear membrane protein emerin.

eLife

Mella J, Volk RF, Zaro BW, Buchwalter A

The nuclear periphery confers repression on H3K9me2-marked genes and transposons to shape cell fate.

Nature cell biology

Marin HC, Allen C, Simental E, Martin EW, Panning B, Al-Sady B, Buchwalter A

Lamin B1 and LAP2β resist cytoskeletal force to maintain lamin A/C meshwork organization and preserve nuclear integrity.

Molecular biology of the cell

Alabi Y, Aksenova V, Arnaoutov A, Marin H, Dasso M, Buchwalter A

C-terminal tagging, transmembrane domain hydrophobicity, and an ER retention motif influence the secretory trafficking of the inner nuclear membrane protein emerin.

bioRxiv : the preprint server for biology

Mella J, Volk R, Zaro B, Buchwalter A

Proteome Birthdating: A Single-Sample Approach for Measuring Global Turnover Dynamics and "Protein Age".

Bio-protocol

Meadow ME, Broas S, Hoare M, Ahmed M, Alimohammadi F, Welle KA, Swovick K, Hryhorenko JR, Jain A, Martinez JC, Seluanov A, Gorbunova V, Buchwalter A, Ghaemmaghami S

Modeling the consequences of age-linked rDNA hypermethylation with dCas9-directed DNA methylation in human cells.

PloS one

Blokhina Y, Buchwalter A

What does it take to build a nucleus?

Nature reviews. Molecular cell biology

Buchwalter A

The nuclear periphery confers repression on H3K9me2-marked genes and transposons to shape cell fate.

bioRxiv : the preprint server for biology

Marin H, Simental E, Allen C, Martin E, Panning B, Al-Sady B, Buchwalter A

Proteome Birthdating Reveals Age-Selectivity of Protein Ubiquitination.

Molecular & cellular proteomics : MCP

Meadow ME, Broas S, Hoare M, Alimohammadi F, Welle KA, Swovick K, Hryhorenko JR, Martinez JC, Biashad SA, Seluanov A, Gorbunova V, Buchwalter A, Ghaemmaghami S

In vivo protein turnover rates in varying oxygen tensions nominate MYBBP1A as a mediator of the hyperoxia response.

Science advances

Chen X, Haribowo AG, Baik AH, Fossati A, Stevenson E, Chen YR, Reyes NS, Peng T, Matthay MA, Traglia M, Pico AR, Jarosz DF, Buchwalter A, Ghaemmaghami S, Swaney DL, Jain IH

Long lifetime and tissue-specific accumulation of lamin A/C in Hutchinson-Gilford progeria syndrome.

The Journal of cell biology

Hasper J, Welle K, Swovick K, Hryhorenko J, Ghaemmaghami S, Buchwalter A

Modeling the consequences of age-linked rDNA hypermethylation with dCas9-directed DNA methylation in human cells.

bioRxiv : the preprint server for biology

Blokhina YP, Buchwalter A

Intermediate, but not average: The unusual lives of the nuclear lamin proteins.

Current opinion in cell biology

Buchwalter A

Long lifetime and selective accumulation of the A-type lamins accounts for the tissue specificity of Hutchinson-Gilford progeria syndrome.

bioRxiv : the preprint server for biology

Hasper J, Welle K, Swovick K, Hryhorenko J, Ghaemmaghami S, Buchwalter A

Turnover and replication analysis by isotope labeling (TRAIL) reveals the influence of tissue context on protein and organelle lifetimes.

Molecular systems biology

Hasper J, Welle K, Hryhorenko J, Ghaemmaghami S, Buchwalter A

Moving fast and breaking things: Incidence and repair of DNA damage within ribosomal DNA repeats.

Mutation research

Blokhina YP, Buchwalter A

Selective clearance of the inner nuclear membrane protein emerin by vesicular transport during ER stress.

eLife

Buchwalter A, Schulte R, Tsai H, Capitanio J, Hetzer M

Coaching from the sidelines: the nuclear periphery in genome regulation.

Nature reviews. Genetics

Buchwalter A, Kaneshiro JM, Hetzer MW

Nucleolar expansion and elevated protein translation in premature aging.

Nature communications

Buchwalter A, Hetzer MW

Access of torsinA to the inner nuclear membrane is activity dependent and regulated in the endoplasmic reticulum.

Journal of cell science

Goodchild RE, Buchwalter AL, Naismith TV, Holbrook K, Billion K, Dauer WT, Liang CC, Dear ML, Hanson PI

Nup50 is required for cell differentiation and exhibits transcription-dependent dynamics.

Molecular biology of the cell

Buchwalter AL, Liang Y, Hetzer MW

Nuclear pores set the speed limit for mitosis.

Cell

Buchwalter A, Hetzer MW

Static retention of the lumenal monotopic membrane protein torsinA in the endoplasmic reticulum.

The EMBO journal

Vander Heyden AB, Naismith TV, Snapp EL, Hanson PI

LULL1 retargets TorsinA to the nuclear envelope revealing an activity that is impaired by the DYT1 dystonia mutation.

Molecular biology of the cell

Vander Heyden AB, Naismith TV, Snapp EL, Hodzic D, Hanson PI

Expression of VACM-1/cul5 mutant in endothelial cells induces MAPK phosphorylation and maspin degradation and converts cells to the angiogenic phenotype.

Microvascular research

Buchwalter A, Van Dort C, Schultz S, Smith R, Le IP, Abbott JL, Oosterhouse E, Johnson AE, Hansen-Smith F, Burnatowska-Hledin M

Estrogen-dependent growth and estrogen receptor (ER)-alpha concentration in T47D breast cancer cells are inhibited by VACM-1, a cul 5 gene.

Molecular and cellular biochemistry

Johnson AE, Le IP, Buchwalter A, Burnatowska-Hledin MA

R7BP augments the function of RGS7*Gbeta5 complexes by a plasma membrane-targeting mechanism.

The Journal of biological chemistry

Drenan RM, Doupnik CA, Jayaraman M, Buchwalter AL, Kaltenbronn KM, Huettner JE, Linder ME, Blumer KJ