Geeta Narlikar, PhD

Professor
Biochemistry and Biophysics
+1 415 514-0394
Publications: 

Satellite repeat transcripts modulate heterochromatin condensates and safeguard chromosome stability in mouse embryonic stem cells.

Nature communications

Novo CL, Wong EV, Hockings C, Poudel C, Sheekey E, Wiese M, Okkenhaug H, Boulton SJ, Basu S, Walker S, Kaminski Schierle GS, Narlikar GJ, Rugg-Gunn PJ

A hexasome is the preferred substrate for the INO80 chromatin remodeling complex, allowing versatility of function.

Molecular cell

Hsieh LJ, Gourdet MA, Moore CM, Muñoz EN, Gamarra N, Ramani V, Narlikar GJ

Histone dynamics play a critical role in SNF2h-mediated nucleosome sliding.

Nature structural & molecular biology

Gamarra N, Narlikar GJ

HP1 proteins compact DNA into mechanically and positionally stable phase separated domains.

eLife

Keenen MM, Brown D, Brennan LD, Renger R, Khoo H, Carlson CR, Huang B, Grill SW, Narlikar GJ, Redding S

Massively multiplex single-molecule oligonucleosome footprinting.

eLife

Abdulhay NJ, McNally CP, Hsieh LJ, Kasinathan S, Keith A, Estes LS, Karimzadeh M, Underwood JG, Goodarzi H, Narlikar GJ, Ramani V

Enzymatic Reactions inside Biological Condensates.

Journal of molecular biology

Zhang Y, Narlikar GJ, Kutateladze TG

Biophysical Properties of HP1-Mediated Heterochromatin.

Cold Spring Harbor symposia on quantitative biology

Sanulli S, Gross JD, Narlikar GJ

Zscan4 binds nucleosomal microsatellite DNA and protects mouse two-cell embryos from DNA damage.

Science advances

Srinivasan R, Nady N, Arora N, Hsieh LJ, Swigut T, Narlikar GJ, Wossidlo M, Wysocka J

Evolutionary Persistence of DNA Methylation for Millions of Years after Ancient Loss of a De Novo Methyltransferase.

Cell

Catania S, Dumesic PA, Pimentel H, Nasif A, Stoddard CI, Burke JE, Diedrich JK, Cooke S, Shea T, Gienger E, Lintner R, Yates JR, Hajkova P, Narlikar GJ, Cuomo CA, Pritchard JK, Madhani HD

Evolutionary Persistence of DNA Methylation for Millions of Years after Ancient Loss of a De Novo Methyltransferase.

Cell

Catania S, Dumesic PA, Pimentel H, Nasif A, Stoddard CI, Burke JE, Diedrich JK, Cook S, Shea T, Geinger E, Lintner R, Yates JR, Hajkova P, Narlikar GJ, Cuomo CA, Pritchard JK, Madhani HD

Phase-separation in chromatin organization.

Journal of biosciences

Narlikar GJ

Topical collection on Chromatin Biology and Epigenetics.

Journal of biosciences

Narlikar G, Galande S

HP1 reshapes nucleosome core to promote heterochromatin phase separation.

Nature

Sanulli S, Trnka MJ, Dharmarajan V, Tibble RW, Pascal BD, Burlingame AL, Griffin PR, Gross JD, Narlikar GJ

Chromatin topology, condensates and gene regulation: shifting paradigms or just a phase?

Development (Cambridge, England)

Mir M, Bickmore W, Furlong EEM, Narlikar G

Cryo-EM structures of remodeler-nucleosome intermediates suggest allosteric control through the nucleosome.

eLife

Armache JP, Gamarra N, Johnson SL, Leonard JD, Wu S, Narlikar GJ, Cheng Y

Ion counting demonstrates a high electrostatic field generated by the nucleosome.

eLife

Gebala M, Johnson SL, Narlikar GJ, Herschlag D

The Role of Phase-Separation in Heterochromatin.

Biophysical journal

Geeta Narlikar, Serena Sanulli, John D. Gross, Patrick Griffin, Mike Trnka

A Nucleosome Bridging Mechanism for Activation of a Maintenance DNA Methyltransferase.

Molecular cell

Stoddard CI, Feng S, Campbell MG, Liu W, Wang H, Zhong X, Bernatavichute Y, Cheng Y, Jacobsen SE, Narlikar GJ

Visualization and Quantitation of Phase-Separated Droplet Formation by Human HP1a.

Methods in enzymology

Keenen MM, Larson AG, Narlikar GJ

The nucleosomal acidic patch relieves auto-inhibition by the ISWI remodeler SNF2h.

eLife

Gamarra N, Johnson SL, Trnka MJ, Burlingame AL, Narlikar GJ

The Yeast INO80 Complex Operates as a Tunable DNA Length-Sensitive Switch to Regulate Nucleosome Sliding.

Molecular cell

Zhou CY, Johnson SL, Lee LJ, Longhurst AD, Beckwith SL, Johnson MJ, Morrison AJ, Narlikar GJ

Dissecting the Mechanism of HP1 Mediated Chromatin Compaction via Single Molecule DNA Curtains.

Biophysical journal

Madeline M. Keenen, Adam G. Larson, Geeta J. Narlikar, Sy Redding

Biochemical Basis for Distinct Roles of the Heterochromatin Proteins Swi6 and Chp2.

Journal of molecular biology

Isaac RS, Sanulli S, Tibble R, Hornsby M, Ravalin M, Craik CS, Gross JD, Narlikar GJ

Liquid droplet formation by HP1a suggests a role for phase separation in heterochromatin.

Nature

Larson AG, Elnatan D, Keenen MM, Trnka MJ, Johnston JB, Burlingame AL, Agard DA, Redding S, Narlikar GJ

Regulation of Rvb1/Rvb2 by a Domain within the INO80 Chromatin Remodeling Complex Implicates the Yeast Rvbs as Protein Assembly Chaperones.

Cell reports

Zhou CY, Stoddard CI, Johnston JB, Trnka MJ, Echeverria I, Palovcak E, Sali A, Burlingame AL, Cheng Y, Narlikar GJ

Mechanisms of ATP-Dependent Chromatin Remodeling Motors.

Annual review of biophysics

Zhou CY, Johnson SL, Gamarra NI, Narlikar GJ

Nucleosome breathing and remodeling constrain CRISPR-Cas9 function.

eLife

Isaac RS, Jiang F, Doudna JA, Lim WA, Narlikar GJ, Almeida R

Single-Molecule Mechanistic Dissection of a Chromatin Remodeling Motor.

Biophysical journal

Stephanie L. Johnson, Nathan I. Gamarra, Matthew J. Johnson, Geeta J. Narlikar

A multilaboratory comparison of calibration accuracy and the performance of external references in analytical ultracentrifugation.

PloS one

Zhao H, Ghirlando R, Alfonso C, Arisaka F, Attali I, Bain DL, Bakhtina MM, Becker DF, Bedwell GJ, Bekdemir A, Besong TM, Birck C, Brautigam CA, Brennerman W, Byron O, Bzowska A, Chaires JB, Chaton CT, Cölfen H, Connaghan KD, Crowley KA, Curth U, Daviter T, Dean WL, Díez AI, Ebel C, Eckert DM, Eisele LE, Eisenstein E, England P, Escalante C, Fagan JA, Fairman R, Finn RM, Fischle W, de la Torre JG, Gor J, Gustafsson H, Hall D, Harding SE, Cifre JG, Herr AB, Howell EE, Isaac RS, Jao SC, Jose D, Kim SJ, Kokona B, Kornblatt JA, Kosek D, Krayukhina E, Krzizike D, Kusznir EA, Kwon H, Larson A, Laue TM, Le Roy A, Leech AP, Lilie H, Luger K, Luque-Ortega JR, Ma J, May CA, Maynard EL, Modrak-Wojcik A, Mok YF, Mücke N, Nagel-Steger L, Narlikar GJ, Noda M, Nourse A, Obsil T, Park CK, Park JK, Pawelek PD, Perdue EE, Perkins SJ, Perugini MA, Peterson CL, Peverelli MG, Piszczek G, Prag G, Prevelige PE, Raynal BD, Rezabkova L, Richter K, Ringel AE, Rosenberg R, Rowe AJ, Rufer AC, Scott DJ, Seravalli JG, Solovyova AS, Song R, Staunton D, Stoddard C, Stott K, Strauss HM, Streicher WW, Sumida JP, Swygert SG, Szczepanowski RH, Tessmer I, Toth RT, Tripathy A, Uchiyama S, Uebel SF, Unzai S, Gruber AV, von Hippel PH, Wandrey C, Wang SH, Weitzel SE, Wielgus-Kutrowska B, Wolberger C, Wolff M, Wright E, Wu YS, Wubben JM, Schuck P

Mechanisms of functional promiscuity by HP1 proteins.

Trends in cell biology

Canzio D, Larson A, Narlikar GJ

The histone H4 tail regulates the conformation of the ATP-binding pocket in the SNF2h chromatin remodeling enzyme.

Journal of molecular biology

Racki LR, Naber N, Pate E, Leonard JD, Cooke R, Narlikar GJ

Mechanism of Nucleosome Remodeling by INO80 from S. Cerevisiae.

Biophysical journal

Coral Y. Zhou, Geeta J. Narlikar

The Mechanism of Nucleosome Spacing by a Dimeric Chromatin Remodeling Enzyme.

Biophysical journal

John D. Leonard, Jean-Paul Armache, Nariman Naber, Shenping Wu, Edward Pate, Roger Cooke, Yifan Cheng, Geeta J. Narlikar

Mechanisms and functions of ATP-dependent chromatin-remodeling enzymes.

Cell

Narlikar GJ, Sundaramoorthy R, Owen-Hughes T

Reconstitution of nucleosome demethylation and catalytic properties of a Jumonji histone demethylase.

Chemistry & biology

Shiau C, Trnka MJ, Bozicevic A, Ortiz Torres I, Al-Sady B, Burlingame AL, Narlikar GJ, Fujimori DG

Mechanistic Analysis of HP1 heterochromatin assembly.

The FASEB Journal

Geeta Narlikar, Daniele Canzio, Maofu Liao, Nariman Naber, Ed Pate, Adam Larson, Shenping Wu, Roger Cooke, Peter Schuck, Yifan Cheng

A conformational switch in HP1 releases auto-inhibition to drive heterochromatin assembly.

Nature

Canzio D, Liao M, Naber N, Pate E, Larson A, Wu S, Marina DB, Garcia JF, Madhani HD, Cooke R, Schuck P, Cheng Y, Narlikar GJ

Ers1 links HP1 to RNAi.

Proceedings of the National Academy of Sciences of the United States of America

Rougemaille M, Braun S, Coyle S, Dumesic PA, Garcia JF, Isaac RS, Libri D, Narlikar GJ, Madhani HD

Analysis of changes in nucleosome conformation using fluorescence resonance energy transfer.

Methods in molecular biology (Clifton, N.J.)

Shahian T, Narlikar GJ

Mechanisms of Chromatin Remodeling Motors.

Biophysical journal

Geeta Narlikar, John Leonard, Lisa Racki, Nariman Naber, Roger Cooke, Yifan Cheng

Structural basis of silencing: Sir3 BAH domain in complex with a nucleosome at 3.0 Å resolution.

Science (New York, N.Y.)

Armache KJ, Garlick JD, Canzio D, Narlikar GJ, Kingston RE

Site-specific acetylation mark on an essential chromatin-remodeling complex promotes resistance to replication stress.

Proceedings of the National Academy of Sciences of the United States of America

Charles GM, Chen C, Shih SC, Collins SR, Beltrao P, Zhang X, Sharma T, Tan S, Burlingame AL, Krogan NJ, Madhani HD, Narlikar GJ

EPR Spectroscopy Shows That the H4 Tail of the Nucleosome Influences the Nucleotide-Binding Pocket of Acf.

Biophysical journal

Lisa Racki, Nariman Naber, Roger Cooke, Geeta Narlikar

Chromodomain-mediated oligomerization of HP1 suggests a nucleosome-bridging mechanism for heterochromatin assembly.

Molecular cell

Canzio D, Chang EY, Shankar S, Kuchenbecker KM, Simon MD, Madhani HD, Narlikar GJ, Al-Sady B

The Chromatin-Remodeling Complex ACF Functions as a Dimeric Motor to Space Nucleosomes.

Biophysical journal

Lisa R. Racki, Geeta J. Narlikar, Roger Cooke, Nariman Naber, Thomas J. Purcell, Janet G. Yang, Yifan Cheng, Ashley Acevedo, Peretz Partensky

Dynamics of nucleosome remodelling by individual ACF complexes.

Nature

Blosser TR, Yang JG, Stone MD, Narlikar GJ, Zhuang X

The chromatin remodeller ACF acts as a dimeric motor to space nucleosomes.

Nature

Racki LR, Yang JG, Naber N, Partensky PD, Acevedo A, Purcell TJ, Cooke R, Cheng Y, Narlikar GJ

Chromatin remodelers act globally, sequence positions nucleosomes locally.

Journal of molecular biology

Partensky PD, Narlikar GJ

Mechanisms of ATP-dependent Chromatin Remodeling Enzymes.

The FASEB Journal

Geeta J Narlikar, Lisa Racki, Janet Yang, Yifan Cheng, Nariman Naber, Roger Cooke

Nucleosome Sliding by ACF is Processive and Bidirectional.

Biophysical journal

Timothy R. Blosser, Michael D. Stone, Janet Yang, Geeta Narlikar, Xiaowei Zhuang

Epigenomics: a roadmap, but to where?

Science (New York, N.Y.)

Madhani HD, Francis NJ, Kingston RE, Kornberg RD, Moazed D, Narlikar GJ, Panning B, Struhl K

ATP-dependent chromatin remodeling enzymes: two heads are not better, just different.

Current opinion in genetics & development

Racki LR, Narlikar GJ

Mechanism of the ATP-dependent chromatin remodeling complex ACF.

The FASEB Journal

Geeta J Narlikar, Janet G Yang, Lisa Racki, Peretz Partensky

The site-specific installation of methyl-lysine analogs into recombinant histones.

Cell

Simon MD, Chu F, Racki LR, de la Cruz CC, Burlingame AL, Panning B, Narlikar GJ, Shokat KM

FRET-based methods to study ATP-dependent changes in chromatin structure.

Methods (San Diego, Calif.)

Yang JG, Narlikar GJ

The chromatin-remodeling enzyme ACF is an ATP-dependent DNA length sensor that regulates nucleosome spacing.

Nature structural & molecular biology

Yang JG, Madrid TS, Sevastopoulos E, Narlikar GJ

Human ACF1 alters the remodeling strategy of SNF2h.

The Journal of biological chemistry

He X, Fan HY, Narlikar GJ, Kingston RE

Heterogeneous nuclear ribonucleoprotein C1/C2, MeCP1, and SWI/SNF form a chromatin remodeling complex at the beta-globin locus control region.

Proceedings of the National Academy of Sciences of the United States of America

Mahajan MC, Narlikar GJ, Boyapaty G, Kingston RE, Weissman SM

Noncovalent modification of chromatin: different remodeled products with different ATPase domains.

Cold Spring Harbor symposia on quantitative biology

Fan HY, Narlikar GJ, Kingston RE

Distinct strategies to make nucleosomal DNA accessible.

Molecular cell

Fan HY, He X, Kingston RE, Narlikar GJ

Nucleosome remodeling by the human SWI/SNF complex requires transient global disruption of histone-DNA interactions.

Molecular and cellular biology

Aoyagi S, Narlikar G, Zheng C, Sif S, Kingston RE, Hayes JJ

Functional differences between the human ATP-dependent nucleosome remodeling proteins BRG1 and SNF2H.

The Journal of biological chemistry

Aalfs JD, Narlikar GJ, Kingston RE

Assembly of nucleosomal templates by salt dialysis.

Current protocols in molecular biology

Lee KM, Narlikar G

Rvb1p and Rvb2p are essential components of a chromatin remodeling complex that regulates transcription of over 5% of yeast genes.

The Journal of biological chemistry

Jónsson ZO, Dhar SK, Narlikar GJ, Auty R, Wagle N, Pellman D, Pratt RE, Kingston R, Dutta A

Stability of a human SWI-SNF remodeled nucleosomal array.

Molecular and cellular biology

Guyon JR, Narlikar GJ, Sullivan EK, Kingston RE

Stable remodeling of tailless nucleosomes by the human SWI-SNF complex.

Molecular and cellular biology

Guyon JR, Narlikar GJ, Sif S, Kingston RE

Reconstitution of a core chromatin remodeling complex from SWI/SNF subunits.

Molecular cell

Phelan ML, Sif S, Narlikar GJ, Kingston RE

Use of binding energy by an RNA enzyme for catalysis by positioning and substrate destabilization.

Proceedings of the National Academy of Sciences of the United States of America

Narlikar GJ, Gopalakrishnan V, McConnell TS, Usman N, Herschlag D