
Professor and Chair
M_Biochemistry and Biophysics
+1 415 514-0394
Publications
A biochemical screening platform to target chromatin states using condensates as a tool.
SLAS discovery : advancing life sciences R & D
Understanding how genetically encoded tags and crowding agents affect phase separation by heterochromatin protein HP1α.
Cell reports methods
Chromatin mimicry by human JC virus.
bioRxiv : the preprint server for biology
ATP-dependent remodeling of chromatin condensates uncovers distinct mesoscale effects of two remodelers.
bioRxiv : the preprint server for biology
HMGB1 restores a dynamic chromatin environment in the presence of linker histone by deforming nucleosomal DNA.
bioRxiv : the preprint server for biology
Functionalized graphene-oxide grids enable high-resolution cryo-EM structures of the SNF2h-nucleosome complex without crosslinking.
Nature communications
Hexasomal particles: consequence or also consequential?
Current opinion in genetics & development
Understanding how genetically encoded tags affect phase separation by Heterochromatin Protein HP1α.
bioRxiv : the preprint server for biology
Nucleosome density shapes kilobase-scale regulation by a mammalian chromatin remodeler.
Nature structural & molecular biology
Catalytic and non-catalytic mechanisms of histone H4 lysine 20 methyltransferase SUV420H1.
Molecular cell
Reorientation of INO80 on hexasomes reveals basis for mechanistic versatility.
Science (New York, N.Y.)
Functionalized graphene-oxide grids enable high-resolution cryo-EM structures of the SNF2h-nucleosome complex without crosslinking.
bioRxiv : the preprint server for biology
In diverse conditions, intrinsic chromatin condensates have liquid-like material properties.
Proceedings of the National Academy of Sciences of the United States of America
Catalytic and non-catalytic mechanisms of histone H4 lysine 20 methyltransferase SUV420H1.
bioRxiv : the preprint server for biology
Satellite repeat transcripts modulate heterochromatin condensates and safeguard chromosome stability in mouse embryonic stem cells.
Nature communications
ATP Hydrolysis Coordinates the Activities of Two Motors in a Dimeric Chromatin Remodeling Enzyme.
Journal of molecular biology
A hexasome is the preferred substrate for the INO80 chromatin remodeling complex, allowing versatility of function.
Molecular cell
A hexasome is the preferred substrate of the INO80 chromatin remodeling complex.
Biophysical journal
Histone dynamics play a critical role in SNF2h-mediated nucleosome sliding.
Nature structural & molecular biology
Generation and Biochemical Characterization of Phase-Separated Droplets Formed by Nucleic Acid Binding Proteins: Using HP1 as a Model System.
Current protocols
Is transcriptional regulation just going through a phase?
Molecular cell
Collaboration through chromatin: mechanisms of molecular motors at the interface of transcription and chromatin structure.
Journal of molecular biology
Enzymatic Reactions inside Biological Condensates.
Journal of molecular biology
Biophysical Properties of HP1-Mediated Heterochromatin.
Cold Spring Harbor symposia on quantitative biology
ATP Hydrolysis by the SNF2 Domain of Dnmt5 Is Coupled to Both Specific Recognition and Modification of Hemimethylated DNA.
Molecular cell
Zscan4 binds nucleosomal microsatellite DNA and protects mouse two-cell embryos from DNA damage.
Science advances
Phase-separation in chromatin organization.
Journal of biosciences
Topical collection on Chromatin Biology and Epigenetics.
Journal of biosciences
Chromatin topology, condensates and gene regulation: shifting paradigms or just a phase?
Development (Cambridge, England)
The Role of Phase-Separation in Heterochromatin.
Biophysical journal
A Nucleosome Bridging Mechanism for Activation of a Maintenance DNA Methyltransferase.
Molecular cell
Visualization and Quantitation of Phase-Separated Droplet Formation by Human HP1a.
Methods in enzymology
The Yeast INO80 Complex Operates as a Tunable DNA Length-Sensitive Switch to Regulate Nucleosome Sliding.
Molecular cell
Dissecting the Mechanism of HP1 Mediated Chromatin Compaction via Single Molecule DNA Curtains.
Biophysical journal
Biochemical Basis for Distinct Roles of the Heterochromatin Proteins Swi6 and Chp2.
Journal of molecular biology
Distortion of histone octamer core promotes nucleosome mobilization by a chromatin remodeler.
Science (New York, N.Y.)
Mechanisms of ATP-Dependent Chromatin Remodeling Motors.
Annual review of biophysics
Analysis of Nucleosome Sliding by ATP-Dependent Chromatin Remodeling Enzymes.
Methods in enzymology
Single-Molecule Mechanistic Dissection of a Chromatin Remodeling Motor.
Biophysical journal
A nucleotide-driven switch regulates flanking DNA length sensing by a dimeric chromatin remodeler.
Molecular cell
Mechanisms of functional promiscuity by HP1 proteins.
Trends in cell biology
The histone H4 tail regulates the conformation of the ATP-binding pocket in the SNF2h chromatin remodeling enzyme.
Journal of molecular biology
Mechanism of Nucleosome Remodeling by INO80 from S. Cerevisiae.
Biophysical journal
The Mechanism of Nucleosome Spacing by a Dimeric Chromatin Remodeling Enzyme.
Biophysical journal
Division of labor between the chromodomains of HP1 and Suv39 methylase enables coordination of heterochromatin spread.
Molecular cell
Reconstitution of nucleosome demethylation and catalytic properties of a Jumonji histone demethylase.
Chemistry & biology
Mechanistic Analysis of HP1 heterochromatin assembly.
The FASEB Journal
Ers1 links HP1 to RNAi.
Proceedings of the National Academy of Sciences of the United States of America
Analysis of changes in nucleosome conformation using fluorescence resonance energy transfer.
Methods in molecular biology (Clifton, N.J.)
Mechanisms of Chromatin Remodeling Motors.
Biophysical journal
Structural basis of silencing: Sir3 BAH domain in complex with a nucleosome at 3.0 Å resolution.
Science (New York, N.Y.)
Site-specific acetylation mark on an essential chromatin-remodeling complex promotes resistance to replication stress.
Proceedings of the National Academy of Sciences of the United States of America
EPR Spectroscopy Shows That the H4 Tail of the Nucleosome Influences the Nucleotide-Binding Pocket of Acf.
Biophysical journal
Chromodomain-mediated oligomerization of HP1 suggests a nucleosome-bridging mechanism for heterochromatin assembly.
Molecular cell
A proposal for kinetic proof reading by ISWI family chromatin remodeling motors.
Current opinion in chemical biology
The Chromatin-Remodeling Complex ACF Functions as a Dimeric Motor to Space Nucleosomes.
Biophysical journal
Chromatin remodelers act globally, sequence positions nucleosomes locally.
Journal of molecular biology
Mechanisms of ATP-dependent Chromatin Remodeling Enzymes.
The FASEB Journal
Nucleosome Sliding by ACF is Processive and Bidirectional.
Biophysical journal
Epigenomics: a roadmap, but to where?
Science (New York, N.Y.)
ATP-dependent chromatin remodeling enzymes: two heads are not better, just different.
Current opinion in genetics & development
Mechanism of the ATP-dependent chromatin remodeling complex ACF.
The FASEB Journal
FRET-based methods to study ATP-dependent changes in chromatin structure.
Methods (San Diego, Calif.)
The chromatin-remodeling enzyme ACF is an ATP-dependent DNA length sensor that regulates nucleosome spacing.
Nature structural & molecular biology
Human ACF1 alters the remodeling strategy of SNF2h.
The Journal of biological chemistry
Heterogeneous nuclear ribonucleoprotein C1/C2, MeCP1, and SWI/SNF form a chromatin remodeling complex at the beta-globin locus control region.
Proceedings of the National Academy of Sciences of the United States of America
Noncovalent modification of chromatin: different remodeled products with different ATPase domains.
Cold Spring Harbor symposia on quantitative biology
Distinct strategies to make nucleosomal DNA accessible.
Molecular cell
Nucleosome remodeling by the human SWI/SNF complex requires transient global disruption of histone-DNA interactions.
Molecular and cellular biology
Generation and interconversion of multiple distinct nucleosomal states as a mechanism for catalyzing chromatin fluidity.
Molecular cell
Functional differences between the human ATP-dependent nucleosome remodeling proteins BRG1 and SNF2H.
The Journal of biological chemistry
Assembly of nucleosomal templates by salt dialysis.
Current protocols in molecular biology
Rvb1p and Rvb2p are essential components of a chromatin remodeling complex that regulates transcription of over 5% of yeast genes.
The Journal of biological chemistry
Stability of a human SWI-SNF remodeled nucleosomal array.
Molecular and cellular biology
ATP-dependent remodeling and acetylation as regulators of chromatin fluidity.
Genes & development
Stable remodeling of tailless nucleosomes by the human SWI-SNF complex.
Molecular and cellular biology
Direct demonstration of the catalytic role of binding interactions in an enzymatic reaction.
Biochemistry
Mechanistic aspects of enzymatic catalysis: lessons from comparison of RNA and protein enzymes.
Annual review of biochemistry
Isolation of a local tertiary folding transition in the context of a globally folded RNA.
Nature structural biology
Use of binding energy by an RNA enzyme for catalysis by positioning and substrate destabilization.
Proceedings of the National Academy of Sciences of the United States of America